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Microbiota and inflammatory bowel diseases
Gajdárová, Zuzana ; Kostovčíková, Klára (advisor) ; Grobárová, Valéria (referee)
Inflammatory bowel diseases (IBD) are an autoimmune illnesses affecting gastrointestinal tract. The main types include ulcerative colitis and Crohn's disease. Recently, primary sclerosing cholangitis (PSC) has also been associated with IBD. PSC is a chronic liver disease associated with bile duct stenosis. The exact pathogenesis and etiology of these diseases is not clear, despite the great efforts of the scientific community. They are multifactorial diseases that are associated with dysbiosis of intestinal microbiota. Their diagnosis is based on for patients unpleasant endoscopic examinations and therefore the search for new serum biomarkers is needed and appreciated target of scientific interest. In the first part of diploma thesis, we focused on the reactivity of peripheral blood cells of IBD patients to 10 selected representatives of typical intestinal microbiota: Lactobacillus plantarum, Bifidobacterium adolescentis, Blautia coccoides, Roseburia intestinalis, Eubacterium rectale, Faecalibacterium prausnitzii, Ruminococcus flavefaciens, Bacteroides thetaiotaomicron, Prevotella ruminicola and Escherichia coli. Reactivity of CD, UC and PSC- IBD patients was increased after stimulation with Faecalibacterium, Lactobacillus and Prevotella. However, we got low percentage of cytokine-producing cells,...
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The significance of morphology in differential diagnosis of lymphoproliferative disorders.
Kaválková, Adriana ; Fátorová, Ilona (advisor) ; Pešková, Eliška (referee)
This work deals with lymphoproliferative diseases in which morphology can contribute to diagnosis and speed up the diagnostic process. The cytomorphological examination is performed whenever any hematopathology is suspected. Cell morphology is assessed from both peripheral blood and bone marrow aspirates. We evaluate many parameters during microscopic examination of the cells: size, shape and deposition of the nucleus, chromatin structure, presence of nuclei, cytoplasm volume and colour, the presence of granules, vacuole, etc. The most common finding in peripheral blood is reactive lymphocytosis and often the presence of cellular atypia: e.g. visible nucleus, a distinct fine structure of chromatin, heterogeneity of the nucleus. Lymphoproliferation can be manifested by non-specific clinical and laboratory findings, which are sometimes difficult to properly evaluate. Therefore should be the results of other laboratory tests and clinical picture taken into account too. Morphology continues to play an important role in the process of recognizing leukaemias and lymphomas. In today's classification responds morphology seldom to the question what disease we looking at. However, the examination is still far more accessible and usually faster than follow-up laboratory methods and has its place in the chain...
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The use of molecular-biology methods (QRT-PCR) and immunocytological methods (flow cytometry and immunocytochemistry) for the detection of minimal residual disease in neuroblastoma
Grüncveigová, Veronika ; Vícha, Aleš (advisor) ; Daňková, Pavlína (referee)
With a continuous development of molecular-biology methods more attention has been paid to molecular detection of minimal residual diseases in solid tumors. In our study we focused on detection of MRD in neuroblastoma. Neuroblastoma is one of the peripheral neuroblastic tumors (pNTs) that accounts approximately for10 percent of all childhood cancers. The question raised however not answered until this day is whether evidence of MRD in bone marrow may be used as independent prognostic factor in diagnosis of neuroblastoma. Furthermore, it is important to establish what kind of testing technique should be used and what values to look at. There exist various methodologies in detection of MRD evidence in neuroblastoma. These methods differ in cost and complexity, but mainly some of them are more specific and sensitive than the other. Cancer cells may be detected in the blood as well as in the bone marrow. Very often it is the bone marrow that is affected by the metastasis in neuroblastoma, therefore 85% of all high risk neuroblastomas show positive results in the standard cytomorphology tests of bone marrow. Low numbers of cancer cells in bone marrow or peripheral blood (especially during or after the end of treatment) are below the standard values of detection limit in most of the classic methodologies...
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Prognostic significance of atypical leukemic cell morphology in chronic lymphocytic leukemia.
Fučíková, Nikola ; Smolej, Lukáš (advisor) ; Vrbacký, Filip (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Health Care Bioanalytics Candidate: Bc. Nikola Fučíková Supervisor: doc. MUDr. Lukáš Smolej, Ph.D. Title of diploma thesis: Prognostic significance of atypical leukemic cell morphology in chronic lymphocytic leukemia The aim of this thesis is to evaluate the prognostic significance of atypical cell morphology and smudge cells in patients with untreated chronic lymphocytic leukemia. We performed differential leukocytes count and classified lymphocytes as typical and atypical in a cohort of 101 patients (median age, 66 years; males, 69%, Rai III/IV stages, 18%). For atypical CLL, we used the 15% threshold and 59% of patients were classified as atypical CLL (aCLL). For smudge cells, we chose the 30% threshold and 33% of patients were classified as smudge cells positive. Patients in early clinical Rai stage (0) had significantly higher number of smudge cells (p=0.04). We didn't find a significant association between aCLL / smudge cells with modern prognostic indicators. We didn't find a relationship between aCLL and the time to first-line therapy (p=0.394). However, patients with aCLL had a significantly shorter overall survival (p=0.0397). There was a trend toward shorter...
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